Pyruvate Kinase, Muscle (PKM) Antikörper

Produktnummer ABIN293067

Dieser Anti- Antikörper ist ein Ziege Polyklonal Antikörper zur Detektion von in WB, IHC, ELISA, IHC (p) und IP. Geeignet für Human und Kaninchen.

Kurzübersicht für Pyruvate Kinase, Muscle (PKM) Antikörper (ABIN293067)

Target: Pyruvate Kinase, Muscle (PKM)

Reaktivität: Human, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails

Spezifität: Pyruvate Kinase (Rabbit Muscle).

Aufreinigung: Purified IgG

Immunogen: Pyruvate kinase purified from rabbit muscle.

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:4000 - 1:20000), IHC, IHC-P (5 μg/mL), IP, WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Rabbit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2 with 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Antigendetails

Target: Pyruvate Kinase, Muscle (PKM)

Andere Bezeichnung: PKM / Pyruvate Kinase, Muscle

Hintergrund: Name/Gene ID: PKM

Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

Gen-ID: 5315

UniProt: P14618

Pathways: Warburg Effekt