Kallikrein 13 Antikörper (AA 19-262)
-
- Target Alle Kallikrein 13 (KLK13) Antikörper anzeigen
- Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))
-
Bindungsspezifität
- AA 19-262
-
Reaktivität
- Human
-
Wirt
- Ziege
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser Kallikrein 13 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunoprecipitation (IP), ELISA, Neutralization (Neut)
- Spezifität
- Selected for its ability to recognize human KLK13 in direct ELISAs and Western Blots. In these formats, this antibody shows ~10 % cross-reactivity with rhKallikrein 8, and less than 5 % cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15.
- Aufreinigung
- Affinity purified
- Immunogen
- NS0-derived rhKLK13 (aa19-262).
- Isotyp
- IgG
- Top Product
- Discover our top product KLK13 Primärantikörper
-
-
- Applikationshinweise
-
Approved: ELISA, IP, Neut, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 8.6 μg/mL (IC50) under conditions in which the enzyme was present at 1 μg/mL and the substrate concentration was 100 M. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 Molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well. - Kommentare
-
Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- deionized water
- Konzentration
- Lot specific
- Buffer
- Lyophilized.
-
- Target
- Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))
- Andere Bezeichnung
- KLK13 / Kallikrein 13 (KLK13 Produkte)
- Synonyme
- KLK-L4 antikoerper, KLKL4 antikoerper, Egfbp-2 antikoerper, mGk-13 antikoerper, kallikrein related peptidase 13 antikoerper, kallikrein related-peptidase 13 antikoerper, KLK13 antikoerper, Klk13 antikoerper
- Hintergrund
-
Name/Gene ID: KLK13
Subfamily: Serine S1
Family: Protease
Synonyms: KLK13, Kallikrein 13, Kallikrein-13, KLK-L4, KLKL4, Kallikrein-like protein 4, Kallikrein-like gene 4 - Gen-ID
- 26085
- UniProt
- Q9UKR3
- Pathways
- Komplementsystem
-