NOTCH4 Antikörper (AA 1778-2003)

Produktnummer ABIN291848

Der Ziege Polyklonal Anti-NOTCH4-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, NOTCH4 in Proben von Human zu detektieren.

Kurzübersicht für NOTCH4 Antikörper (AA 1778-2003) (ABIN291848)

Target: NOTCH4 (Notch 4 (NOTCH4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NOTCH4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-NOTCH4 Antikörper

Bindungsspezifität: AA 1778-2003

Spezifität: Recognizes human Notch4.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived rhNotch-4 (aa1778-2003).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Notch4 in serum starved HUVEC cells stimulated with Jagged 1. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 0.3 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Andere Bezeichnung: NOTCH4

Hintergrund: Name/Gene ID: NOTCH4

Synonyms: NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4

Gen-ID: 4855

UniProt: Q99466

Pathways: Notch Signalweg