Histone H2A Antikörper (AA 1-15)

Produktnummer ABIN291582

Dieser Anti-Histone H2A Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von Histone H2A in WB, IHC und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für Histone H2A Antikörper (AA 1-15) (ABIN291582)

Target: Histone H2A

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Histone H2A Antikörper

Bindungsspezifität: AA 1-15

Spezifität: A 14 kD band is observed.

Homologie: Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Elephant, Bovine, Chicken (100%).

Aufreinigung: Protein G purified

Immunogen: This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acid residues 1-15 (MSGRGKQGGKARAKA) and 81-96 (PRHLQLAIRNDEELNK) of human Histone H2A, GenBank no. ref|NP_066390.1|. Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Elephant, Bovine, Chicken (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu Histone H2A

Target: Histone H2A

NCBI Accession: NP_066390