Vitronectin Antikörper

Produktnummer ABIN2887824

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Vitronectin in WB, ELISA, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh), Hund, Ziege, Pferd und Cat.

Kurzübersicht für Vitronectin Antikörper (ABIN2887824)

Target: Vitronectin (VTN)

Reaktivität: Human, Rind (Kuh), Hund, Ziege, Pferd, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vitronectin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Vitronectin Antikörper

Marke: IHC-plus™

Spezifität: Recognizes human vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Species Crossreactivity: feline, canine, goat, bovine and to a lesser extent with equine. Binds nearly as well to native vitronectin as to denatured. Is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.

Aufreinigung: Affinity purified

Immunogen: Human vitronectin purified from plasma by heparin-affinity chromatography.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:30000), IHC, IHC-P (2.5 μg/mL), WB (1:50)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. ELISA: 1:15000. Coat with vitronectin directly onto the microtiter well. Please note that cross-reactivity with species other than human has not been confirmed in IHC at this time. In the absence of testing data we cannot guarantee cross-reactivity with non-human species in IHC.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 500 mM sodium chloride, 15 mM sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Vitronectin

Target: Vitronectin (VTN)

Andere Bezeichnung: VTN / Vitronectin

Hintergrund: Name/Gene ID: VTN
Family: Matrix Protein

Synonyms: VTN, Epibolin, Serum-spreading factor, VNT, S-protein, Somatomedin B, V75, VN, Complement S-protein, Serum spreading factor, Vitronectin

Gen-ID: 7448

UniProt: P04004

Pathways: Autophagie, Smooth Muscle Cell Migration