Protein A Antikörper (Biotin)

Produktnummer ABIN288033

Dieser Anti-Protein A-Antikörper ist ein Ziege-Polyklonal-Antikörper zum Nachweis von Protein A in ELISA, WB und IHC. Geeignet für Staphylococcus aureus.

Kurzübersicht für Protein A Antikörper (Biotin) (ABIN288033)

Target: Protein A

Reaktivität: Staphylococcus aureus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Protein A Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Protein A Antikörper (Biotin)

Aufreinigung: Protein A affinity chromatography

Immunogen: Protein A antibody (biotin) was raised in goat using Protein A [Staphylococcus aureus] as the immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: IgG fraction supplied as a liquid in 20 mM Potassium Phosphate and 150 M NaCl, pH 7.2, with 10 g/mL BSA (IgG and Protease free) as a stabilizer, and 0.01 % NaN3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Details zu Protein A

Target: Protein A

Hintergrund: Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs. It binds with the Fc region of immunoglobulins through interaction with the heavy chain.