Choline Acetyltransferase Antikörper (N-Term)

Produktnummer ABIN2869094

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Choline Acetyltransferase in WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Choline Acetyltransferase Antikörper (N-Term) (ABIN2869094)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-Choline Acetyltransferase Antikörper

Bindungsspezifität: N-Term

Spezifität: Predicted molecular weight at ~82.5 kDa. Observed molecular weights between 68-70 kDa.

Kreuzreaktivität: Human, Maus

Aufreinigung: Peptide Affinity Purified

Immunogen: Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN2869094 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: Choline Acetyltransferase

Hintergrund: Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).

Gen-ID: 1103

NCBI Accession: NP_001136401

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development