HYOU1 Antikörper (Atto 488)

Produktnummer ABIN2868630

Der Maus Monoklonal Anti-HYOU1-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, HYOU1 in Proben von Human zu detektieren.

Kurzübersicht für HYOU1 Antikörper (Atto 488) (ABIN2868630)

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HYOU1 Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 6E3-2C3

Produktdetails anti-HYOU1 Antikörper (Atto 488)

Spezifität: Detects ~170 kDa.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Recombinant Full length GRP170 Protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2868630 was sufficient for detection of GRP170 in 20 μg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Andere Bezeichnung: GRP170

Hintergrund: GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).

Gen-ID: 10525

NCBI Accession: NP_006389

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interaktom