S1PR1 Antikörper (C-Term)

Produktnummer ABIN2857018

Dieses unabhängig validierte Kaninchen Polyklonal-Antikörper erkennt spezifisch S1PR1 in WB, IF, IHC (p) und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für S1PR1 Antikörper (C-Term) (ABIN2857018)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S1PR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-S1PR1 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit Polyclonal antibody to EDG1 (sphingosine-1-phosphate receptor 1)
EDG1 antibody [C2C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDG1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HepG2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #100014 (Immunofluorescence)

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100014

Datum: 11.05.2016

Antigen: Sphingosine-1-Phosphate Receptor 1 (S1PR1)

Chargennummer: 39471

Validierte Anwendung: Immunofluorescence

Positivkontrolle: Wild-type mouse adult brain cryo-sections

Negativkontrolle: Rabbit IgG

Bewertung: The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.

Validierungsbilder

IF validation of S1PR1 antibody ABIN2857018 on mouse brain sections. A. ABIN2857018 diluted 1:200 (red), DAPI counterstaining (blue). B. Dilution factor 1:100. C. Dilution factor 1:200. D. Dilution factor 1:400.

Protokoll

Primärantikörper: ABIN2857018

Sekundärantikörper: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• • Dry slides at 50-55°C for 15-30min (or RT for 30min).
  • Wash with PBS 5min X 3 (shake gently).
  • Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
  • Incubate with ABIN2857018 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
  • Wash with PBS 5min X 3.
  • Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
  • Wash with PBS 5min X 3.
  • Add three drops of water-based Immu-mount.
  • Cover with 24 X 60 cover glass.
• Imaging:
• • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Anmerkungen: ABIN2857018 worked the best at 1:200. At 1:400, or 1:800, it seems to lose some signals in some cells.

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Andere Bezeichnung: sphingosine-1-phosphate receptor 1

Hintergrund: The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion.

Cellular Localization: Cell membrane, Multi-pass membrane protein

Molekulargewicht: 43 kDa

Gen-ID: 1901

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2