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Villin 1 Antikörper (N-Term)

Der Kaninchen Polyklonal Anti-Villin 1-Antikörper wurde für WB, IF, IHC (p), ICC und IHC (fro) validiert. Er ist geeignet, Villin 1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN2856719

Kurzübersicht für Villin 1 Antikörper (N-Term) (ABIN2856719)

Target

Alle Villin 1 (VIL1) Antikörper anzeigen
Villin 1 (VIL1)

Reaktivität

  • 120
  • 25
  • 20
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 60
  • 60
Kaninchen

Klonalität

  • 82
  • 38
Polyklonal

Konjugat

  • 58
  • 8
  • 6
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
Dieser Villin 1 Antikörper ist unkonjugiert

Applikation

  • 74
  • 50
  • 43
  • 20
  • 18
  • 13
  • 6
  • 5
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Bindungsspezifität

    • 58
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Kreuzreaktivität

    Cat, Hund, Human, Maus, Ratte

    Produktmerkmale

    Rabbit Polyclonal antibody to Villin (villin 1)
    Villin antibody

    Aufreinigung

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the N-terminus region of human Villin. The exact sequence is proprietary.

    Isotyp

    IgG
  • Applikationshinweise

    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Kommentare

    Positive Control: rat kidney , mouse kidney , HepG2

    Validation: Comparison, Orthogonal

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.85 mg/mL

    Buffer

    1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Reed, Luissint, Azcutia, Fan, OLeary, Quiros, Brazil, Nusrat, Parkos: "Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo." in: Nature communications, Vol. 10, Issue 1, pp. 5004, (2019) (PubMed).

  • Target

    Villin 1 (VIL1)

    Andere Bezeichnung

    villin 1

    Hintergrund

    This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed, they result from utilization of alternate poly-adenylation signals present in the terminal exon.

    Cellular Localization: Cytoplasm , cytoskeleton

    Molekulargewicht

    93 kDa

    Gen-ID

    7429

    UniProt

    P09327

    Pathways

    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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