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TJP1 Antikörper (N-Term)

Name: TJP1 Antikörper (N-Term)
Brand: antibodies-online
SKU: ABIN2856624
Price: 659.23 EUR
Availability: InStock

KO Validated TJP1 Reaktivität: Human WB, IP, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

WB Image Sample(30 ug whole cell lysate) A:H1299 5% SDS PAGE antibody diluted at 1:3000 (TJP1 Antikörper (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1, antibody at 1:250 dilution. (TJP1 Antikörper (N-Term))

IHC

WB Image Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term , diluted at 1:500. (TJP1 Antikörper (N-Term))

IHC-P Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. (TJP1 Antikörper (N-Term))

IHC

ICC/IF Image ZO-1 antibody [N1N2], N-term detects TJP1 protein at junction by confocal immunofluorescent analysis. (TJP1 Antikörper (N-Term))

IHC-P Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis. (TJP1 Antikörper (N-Term))

IHC

ICC/IF Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis. (TJP1 Antikörper (N-Term))

Identification of SLC26A3 as a CFTR and ZO-1 binding partner and effects on their interaction. (TJP1 Antikörper (N-Term))

8 Abbildungen

(1 reference)

Produktnummer ABIN2856624

Datenblatt (PDF)

Target Alle TJP1 Antikörper anzeigen

TJP1 (Tight Junction Protein 1 (TJP1))
Bindungsspezifität
15

6

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reaktivität
58

36

34

18

17

8

5

5

3

3

1

1

1

1

1

1

1

1
Human
Wirt
54

3

2

1

1
Kaninchen
Passende Sekundärantikörper
Klonalität
55

6
Polyklonal
Konjugat
38

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser TJP1 Antikörper ist unkonjugiert
Applikation
50

22

19

17

15

13

12

11

9

8

8

2

2

2

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Kreuzreaktivität

Human, Maus

Produktmerkmale

Rabbit Polyclonal antibody to ZO-1 (tight junction protein 1 (zona occludens 1))
ZO-1 antibody [N1N2], N-term

Aufreinigung

Purified by antigen-affinity chromatography.

Güteklasse

KO Validated

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Isotyp

IgG

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anti-Tight Junction Protein 1 (TJP1) (C-Term) antibody
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anti-Tight Junction Protein 1 (TJP1) (Internal Region) antibody
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anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 Reaktivität: Human WB, IHC, ELISA, IF, FACS, FM, MA Wirt: Kaninchen Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
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TJP1 Reaktivität: Human WB, IF Wirt: Maus Monoclonal 1-ZO unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1178-1527) antibody
TJP1 Reaktivität: Human, Maus, Ratte WB, IHC, IF, FACS, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1570-1600), (C-Term) antibody
TJP1 Reaktivität: Human WB, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB15419 unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 Reaktivität: Maus WB, IHC, IP Wirt: Ratte Monoclonal unconjugated

Applikationshinweise

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare

Positive Control: HeLa
Validation: KO/KD

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.22 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Zhang, Heruth, Wu, Zhang, Nsumu, Shortt, Li, Jiang, Wang, Friesen, Li, Ye: "Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models." in: Cell & bioscience, Vol. 9, pp. 40, (2019) (PubMed).
Target

TJP1 (Tight Junction Protein 1 (TJP1))

Andere Bezeichnung

tight junction protein 1 (TJP1 Produkte)

Hintergrund

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.

Cellular Localization: Cell membrane, Peripheral membrane protein, Cytoplasmic side , Cell junction , tight junction

Molekulargewicht

195 kDa

Gen-ID

7082

UniProt

Q07157

Pathways

Carbohydrate Homeostasis, Cell-Cell Junction Organization