DENND1B Antikörper (Center)

Produktnummer ABIN2856495

Dieses Anti-DENND1B-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DENND1B in WB, ICC und IF. Geeignet für Human und Maus.

Kurzübersicht für DENND1B Antikörper (Center) (ABIN2856495)

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DENND1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-DENND1B Antikörper

Bindungsspezifität: Center

Produktmerkmale: Rabbit Polyclonal antibody to DENND1B (DENN/MADD domain containing 1B)
DENND1B antibody [N1C1]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DENND1B. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.9 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu DENND1B

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Andere Bezeichnung: DENND1B

Hintergrund: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2, see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM]

Molekulargewicht: 48 kDa

Gen-ID: 163486