RASGRP4 Antikörper (N-Term)

Produktnummer ABIN2855910

Dieses Anti-RASGRP4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RASGRP4 in WB, IHC (p), IF und ICC. Geeignet für Human.

Kurzübersicht für RASGRP4 Antikörper (N-Term) (ABIN2855910)

Target: RASGRP4 (RAS Guanyl Releasing Protein 4 (RASGRP4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RASGRP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-RASGRP4 Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Human

Produktmerkmale: Rabbit Polyclonal antibody to RASGRP4 (RAS guanyl releasing protein 4)
RASGRP4 antibody [N1N2], N-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RASGRP4. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Jurkat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 40 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu RASGRP4

Target: RASGRP4 (RAS Guanyl Releasing Protein 4 (RASGRP4))

Andere Bezeichnung: RAS guanyl releasing protein 4

Hintergrund: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , Cell membrane

Molekulargewicht: 75 kDa

Gen-ID: 115727

UniProt: Q8TDF6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling