RFC4 Antikörper

Produktnummer ABIN2855757

Dieses Anti-RFC4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RFC4 in WB, IF, IHC (p) und ICC. Geeignet für Human.

Kurzübersicht für RFC4 Antikörper (ABIN2855757)

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-RFC4 Antikörper

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit Polyclonal antibody to RFC4 (replication factor C (activator 1) 4, 37 kDa)
RFC4 antibody [N1C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RFC4. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Mouse liver , 293T , A431 , HeLa , HepG2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.62 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu RFC4

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Andere Bezeichnung: replication factor C subunit 4

Hintergrund: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Cellular Localization: Nucleus

Molekulargewicht: 40 kDa

Gen-ID: 5984

UniProt: P35249

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA