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Pyruvate Dehydrogenase E1 alpha (Center) Antikörper

Reaktivität: Human, Maus, Ratte WB, IF, ICC, IP, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2855747
  • Target
    Pyruvate Dehydrogenase E1 alpha
    Bindungsspezifität
    • 1
    • 1
    Center
    Reaktivität
    Human, Maus, Ratte
    Wirt
    • 2
    Kaninchen
    Klonalität
    • 2
    Polyklonal
    Konjugat
    • 2
    Unkonjugiert
    Applikation
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Kreuzreaktivität
    Rhesusaffen, Schimpanse
    Kreuzreaktivität (Details)
    Rhesus Monkey (100 %), Chimpanzee (100 %)
    Produktmerkmale
    Rabbit Polyclonal antibody to Pyruvate Dehydrogenase E1 alpha (pyruvate dehydrogenase (lipoamide) alpha 1)
    Pyruvate Dehydrogenase E1 alpha antibody
    Aufreinigung
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 alpha. The exact sequence is proprietary.
    Isotyp
    IgG
  • Applikationshinweise
    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*
    Kommentare

    Positive Control: HepG2 , Molt-4 , mouse brain , rat brain

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    Konservierungsmittel
    Thimerosal (Merthiolate)
    Vorsichtsmaßnahmen
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Chao, Wroblewski, Ilkayeva, Stevens, Bain, Meyer, Schenk, Martinez, Vergnes, Narkar, Drew, Hong, Boyadjian, Hevener, Evans, Reue, Spencer, Newgard, Tontonoz: "Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization." in: Journal of lipid research, Vol. 53, Issue 12, pp. 2610-9, (2012) (PubMed).

  • Target
    Pyruvate Dehydrogenase E1 alpha
    Hintergrund
    The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

    Cellular Localization: Mitochondrion matrix
    Molekulargewicht
    43 kDa
    Gen-ID
    5160
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