Pyruvate Dehydrogenase E1 alpha (AA 39-295) Antikörper

Produktnummer ABIN1886427

Der Kaninchen Polyklonal anti-Pyruvate Dehydrogenase E1 alpha Antikörper (ABIN1886427) detektiert spezifisch Pyruvate Dehydrogenase E1 alpha in WB und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Pyruvate Dehydrogenase E1 alpha (AA 39-295) Antikörper (ABIN1886427)

Target: Pyruvate Dehydrogenase E1 alpha

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails

Bindungsspezifität: AA 39-295

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 295 of Pyruvate Dehydrogenase E1 alpha

Anwendungsinformationen

Applikationshinweise: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunofluorescence: 1.100-1.200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: Biohazard Informations: This product contains thimerosal which is hazardous.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Antigendetails

Target: Pyruvate Dehydrogenase E1 alpha

Hintergrund: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA.The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4).The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits.The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

Molekulargewicht: 43 kDa

Gen-ID: 5160

NCBI Accession: NM_000284, NP_000275