Pyruvate Dehydrogenase E1 alpha (Center) Antikörper

Produktnummer ABIN2855747

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB, IF, IP, ICC und IHC (p) validiert. Er ist geeignet, in Proben von Human, Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Pyruvate Dehydrogenase E1 alpha (Center) Antikörper (ABIN2855747)

Target: Pyruvate Dehydrogenase E1 alpha

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: Center

Kreuzreaktivität: Rhesusaffen, Schimpanse

Kreuzreaktivität (Details): Rhesus Monkey (100 %), Chimpanzee (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to Pyruvate Dehydrogenase E1 alpha (pyruvate dehydrogenase (lipoamide) alpha 1)
Pyruvate Dehydrogenase E1 alpha antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 alpha. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Kommentare:

Positive Control: HepG2 , Molt-4 , mouse brain , rat brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen

Chao, Wroblewski, Ilkayeva, Stevens, Bain, Meyer, Schenk, Martinez, Vergnes, Narkar, Drew, Hong, Boyadjian, Hevener, Evans, Reue, Spencer, Newgard, Tontonoz: "Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization." in: Journal of lipid research, Vol. 53, Issue 12, pp. 2610-9, (2012) (PubMed).

Antigendetails

Target: Pyruvate Dehydrogenase E1 alpha

Hintergrund: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

Cellular Localization: Mitochondrion matrix

Molekulargewicht: 43 kDa

Gen-ID: 5160