Pyruvate Dehydrogenase E1 alpha (Center) Antikörper

Produktnummer ABIN2855747

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-Pyruvate Dehydrogenase E1 alpha Antikörper wird verwendet zum Nachweis von Pyruvate Dehydrogenase E1 alpha in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF, IP, ICC und IHC (p).

Kurzübersicht für Pyruvate Dehydrogenase E1 alpha (Center) Antikörper (ABIN2855747)

Target: Pyruvate Dehydrogenase E1 alpha

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: Center

Kreuzreaktivität: Rhesusaffen, Schimpanse

Kreuzreaktivität (Details): Rhesus Monkey (100 %), Chimpanzee (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to Pyruvate Dehydrogenase E1 alpha (pyruvate dehydrogenase (lipoamide) alpha 1)
Pyruvate Dehydrogenase E1 alpha antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 alpha. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Kommentare:

Positive Control: HepG2 , Molt-4 , mouse brain , rat brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen

Chao, Wroblewski, Ilkayeva, Stevens, Bain, Meyer, Schenk, Martinez, Vergnes, Narkar, Drew, Hong, Boyadjian, Hevener, Evans, Reue, Spencer, Newgard, Tontonoz: "Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization." in: Journal of lipid research, Vol. 53, Issue 12, pp. 2610-9, (2012) (PubMed).

Antigendetails

Target: Pyruvate Dehydrogenase E1 alpha

Hintergrund: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

Cellular Localization: Mitochondrion matrix

Molekulargewicht: 43 kDa

Gen-ID: 5160