LIG3 Antikörper (C-Term)

Produktnummer ABIN2855575

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LIG3 in WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für LIG3 Antikörper (C-Term) (ABIN2855575)

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIG3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Güteklasse: KO Validated

Produktdetails anti-LIG3 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Hamster, Human

Produktmerkmale: Rabbit Polyclonal antibody to DNA ligase III (ligase III, DNA, ATP-dependent)
DNA ligase III antibody [C2C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase III. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T

Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.15 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-LIG3 Antikörper (ABIN2855575)

Abdou, Poirier, Hendzel, Weinfeld: "DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair." in: Nucleic acids research, Vol. 43, Issue 2, pp. 875-92, (2015) (PubMed).

Details zu LIG3

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Andere Bezeichnung: DNA ligase 3

Hintergrund: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Cellular Localization: Nucleus

Molekulargewicht: 113 kDa

Gen-ID: 3980

UniProt: P49916

Pathways: DNA Reparatur