NRF1 Antikörper

Produktnummer ABIN2855569

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NRF1 in WB, IHC (p), IF, ICC und IP. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für NRF1 Antikörper (ABIN2855569)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NRF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-NRF1 Antikörper

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to NRF1 (nuclear respiratory factor 1)
NRF1 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NRF1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T nuclear , A431 nuclear , HeLa nuclear , HepG2 nuclear

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.32 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-NRF1 Antikörper (ABIN2855569)

Mesquita, Lamb, Parry, Moore, Smith, Vann, Osburn, Fox, Ruple, Huggins, Fruge, Young, Kavazis, Roberts: "Acute and chronic effects of resistance training on skeletal muscle markers of mitochondrial remodeling in older adults." in: Physiological reports, Vol. 8, Issue 15, pp. e14526, (2020) (PubMed).

Ma, Cheng, Mu, Geng, Zhao, Luo, Ma, Chang, Liu, Gao, Nie, Xie, Han, Chen, Ma, Zhu, Chen: "The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression." in: Nature communications, Vol. 10, Issue 1, pp. 1034, (2019) (PubMed).

Details zu NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Andere Bezeichnung: nuclear respiratory factor 1

Hintergrund: This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Cellular Localization: Nucleus

Molekulargewicht: 54 kDa

Gen-ID: 4899

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha