MDC1 Antikörper

Produktnummer ABIN2855454

Dieses Anti-MDC1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MDC1 in WB, IF, IHC (p) und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für MDC1 Antikörper (ABIN2855454)

Target: MDC1 (Mdc1 (MDC1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-MDC1 Antikörper

Kreuzreaktivität: Human

Produktmerkmale: Rabbit Polyclonal antibody to MDC1 (mediator of DNA-damage checkpoint 1)
MDC1 antibody [N2N3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MDC1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HeLa nucleus

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-MDC1 Antikörper (ABIN2855454)

Burger, Schlackow, Gullerova: "Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks." in: Nucleic acids research, Vol. 47, Issue 7, pp. 3467-3484, (2019) (PubMed).

Chen, Tsai, Wang, Teng: "SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression." in: Scientific reports, Vol. 7, Issue 1, pp. 3842, (2017) (PubMed).

Details zu MDC1

Target: MDC1 (Mdc1 (MDC1))

Andere Bezeichnung: mediator of DNA damage checkpoint 1

Hintergrund: The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Cellular Localization: Nucleus

Molekulargewicht: 227 kDa

Gen-ID: 9656

UniProt: Q14676