PHGDH Antikörper (N-Term)

Produktnummer ABIN2855237

Der Kaninchen Polyklonal Anti-PHGDH-Antikörper wurde für WB, IHC (p), IF, IP, IHC (fro) und ICC validiert. Er ist geeignet, PHGDH in Proben von Human zu detektieren.

Kurzübersicht für PHGDH Antikörper (N-Term) (ABIN2855237)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHGDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Güteklasse: KO Validated

Produktdetails anti-PHGDH Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)
PHGDH antibody [N1N2], N-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Mouse brain , 293T , A431 , HeLaS3 , HeLa

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.87 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Andere Bezeichnung: phosphoglycerate dehydrogenase

Hintergrund: 3-Phosphoglycerate dehydrogenase (PHGDH, EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Molekulargewicht: 57 kDa

Gen-ID: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effekt