PSMA7 Antikörper (Center)

Produktnummer ABIN2855151

Dieses Anti-PSMA7-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PSMA7 in WB und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für PSMA7 Antikörper (Center) (ABIN2855151)

Target: PSMA7 (Proteasome Subunit alpha 7 (PSMA7))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PSMA7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PSMA7 Antikörper

Bindungsspezifität: Center

Kreuzreaktivität: Huhn, Schwein, Ratte (Rattus), Rind (Kuh), Xenopus tropicalis

Kreuzreaktivität (Details): Chicken (96 %), Pig (98 %), Rat (98 %), Bovine (99 %), Xenopus tropicalis (97 %)

Produktmerkmale: Rabbit Polyclonal antibody to PSMA7 (proteasome (prosome, macropain) subunit, alpha type, 7)
PSMA7 antibody [N1C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 7. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.32 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu PSMA7

Target: PSMA7 (Proteasome Subunit alpha 7 (PSMA7))

Andere Bezeichnung: PSMA7

Hintergrund: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9.

Cellular Localization: Cytoplasm , Nucleus

Molekulargewicht: 28 kDa

Gen-ID: 5688

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA