ADAR Antikörper

Produktnummer ABIN2855100

Dieses Anti-ADAR-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ADAR in WB, IF, IP, IHC (p) und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für ADAR Antikörper (ABIN2855100)

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADAR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Güteklasse: KO Validated

Produktdetails anti-ADAR Antikörper

Kreuzreaktivität: Human, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to ADAR1 (adenosine deaminase, RNA-specific)
ADAR1 antibody [N3C1], Internal

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ADAR1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.82 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-ADAR Antikörper (ABIN2855100)

Stejskal, Rájecká, Covelo-Molares, Sinigaglia, Brožinová, Kašiarová, Dohnálková, Reyes-Gutierrez, Cahová, Keegan, O'Connell, Vaňáčová: "Global analysis by LC-MS/MS of N6-methyladenosine and inosine in mRNA reveal complex incidence." in: RNA (New York, N.Y.), Vol. 31, Issue 4, pp. 514-528, (2025) (PubMed).

Details zu ADAR

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Andere Bezeichnung: adenosine deaminase RNA specific

Hintergrund: This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Cellular Localization: Cytoplasm , Nucleus , nucleolus

Molekulargewicht: 136 kDa

Gen-ID: 103

UniProt: P55265

Pathways: Protein targeting to Nucleus