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KLF4 Antikörper (C-Term)

Name: KLF4 Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN2855075
Price: 765 EUR
Availability: InStock

Der Kaninchen Polyklonal Anti-KLF4-Antikörper wurde für WB, FACS und IP validiert. Er ist geeignet, KLF4 in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

WB Image Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE antibody diluted at 1:3000 (KLF4 Antikörper (C-Term))

IP Image Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 μg of KLF4 antibody, Western blot analysis was performed using KLF4 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (KLF4 Antikörper (C-Term))

WB Image Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE antibody diluted at 1:5000 (KLF4 Antikörper (C-Term))

FACS

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000 (KLF4 Antikörper (C-Term))

WB Image HCT116 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody , diluted at 1:2000. (KLF4 Antikörper (C-Term))

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE antibody diluted at 1:3000 (KLF4 Antikörper (C-Term))

DDX3 suppresses stemness gene signature. (KLF4 Antikörper (C-Term))

8 Abbildungen

(1 reference)

Produktnummer ABIN2855075

Kurzübersicht für KLF4 Antikörper (C-Term) (ABIN2855075)

Target

Alle KLF4 Antikörper anzeigen

KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reaktivität

Human, Maus

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser KLF4 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Bindungsspezifität
12

9

8

7

6

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term

Kreuzreaktivität

Ratte (Rattus), Katze, Schwein, Schaf, Rhesusaffen, Rind (Kuh)

Kreuzreaktivität (Details)

Rat (100 %), Cat (100 %), Pig (100 %), Sheep (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Produktmerkmale

Rabbit Polyclonal antibody to KLF4 (Kruppel-like factor 4 (gut))
KLF4 antibody

Aufreinigung

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary.

Isotyp

IgG
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibody
KLF4 Reaktivität: Human WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB14263 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Monoclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reaktivität: Human WB, ELISA, IHC, IF, IP Wirt: Kaninchen Monoclonal 3A1 unconjugated Recombinant Antibody

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 221-320) antibody
KLF4 Reaktivität: Human WB, ELISA, IF Wirt: Maus Monoclonal X1 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (C-Term) antibody
KLF4 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Hund, Kaninchen, Meerschweinchen, Zebrafisch (Danio rerio) WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibody
KLF4 Reaktivität: Human, Maus WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 481-504) antibody
KLF4 Reaktivität: Human WB, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reaktivität: Human WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB17718 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 481-504) antibody
KLF4 Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal RB18934 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reaktivität: Human, Maus WB, IF, FACS Wirt: Maus Monoclonal 56CT5-1-6 unconjugated

Applikationshinweise

Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Kommentare

Positive Control: A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , human ESC , mouse ESC , HCT116 , HCT116 nuclear extract , *MDM-MB-231

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 mg/mL

Buffer

1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel

Thimerosal (Merthiolate)

Vorsichtsmaßnahmen

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Nishimura, Okamoto, Shibue, Mizuta, Shibayama, Yoshino, Murakami, Yamaguchi, Tanaka, Toida, Igarashi: "KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation." in: PLoS ONE, Vol. 15, Issue 2, pp. e0229744, (2020) (PubMed).
Target

KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Andere Bezeichnung

KLF4

Hintergrund

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Cellular Localization: Nucleus

Molekulargewicht

55 kDa

Gen-ID

9314

Pathways

Peptide Hormone Metabolism, Stem Cell Maintenance