Glucocorticoid Receptor Antikörper (N-Term)

Produktnummer ABIN2854939

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Glucocorticoid Receptor in WB, IF, IP, ICC und IHC (p). Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Glucocorticoid Receptor Antikörper (N-Term) (ABIN2854939)

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucocorticoid Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: KO Validated

Produktdetails anti-Glucocorticoid Receptor Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to Glucocorticoid receptor (nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor))
Glucocorticoid receptor antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Glucocorticoid Receptor. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: A431 , mouse heart , rat heart , HeLa

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-Glucocorticoid Receptor Antikörper (ABIN2854939)

Bolt, Stossi, Callison, Mancini, Dandekar, Mancini: "Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-α protein levels and activity." in: Oncogene, Vol. 34, Issue 2, pp. 154-64, (2015) (PubMed).

Bolt, Stossi, Newberg, Orjalo, Johansson, Mancini: "Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses." in: Nucleic acids research, Vol. 41, Issue 7, pp. 4036-48, (2013) (PubMed).

Details zu Glucocorticoid Receptor

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Andere Bezeichnung: nuclear receptor subfamily 3 group C member 1

Hintergrund: The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.

Cellular Localization: Cytoplasm , Nucleus

Molekulargewicht: 86 kDa

Gen-ID: 2908

UniProt: P04150

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process