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VCP Antikörper (C-Term)

Name: VCP Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN2854929
Price: 659.23 EUR
Availability: InStock

Dieses Anti-VCP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von VCP in WB, IF, IP, IHC (p), ICC, IHC (fro) und IHC (wm). Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

WB Image VCP antibody detects VCP protein by western blot analysis. (VCP Antikörper (C-Term))

ICC/IF Image VCP antibody detects VCP protein at cytoplasm and nucleus by immunofluorescent analysis. (VCP Antikörper (C-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using VCP, antibody at 1:500 dilution. (VCP Antikörper (C-Term))

IHC

IP Image Immunoprecipitation of VCP protein from HeLa whole cell extracts using 5 μg of VCP antibody, Western blot analysis was performed using VCP antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (VCP Antikörper (C-Term))

WB Image Sample(30 μg of whole cell lysate) A:A431, B:H1299 7. (VCP Antikörper (C-Term))

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7. (VCP Antikörper (C-Term))

Study of cellular SIL1 immunoreactivity and presence of ER-stress in Hek293 cells overexpressing SIL1 or its variant forms. (VCP Antikörper (C-Term))

8 Abbildungen

(1 reference)

Produktnummer ABIN2854929

Kurzübersicht für VCP Antikörper (C-Term) (ABIN2854929)

Target

Alle VCP Antikörper anzeigen

VCP (Valosin Containing Protein (VCP))

Reaktivität

Human

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser VCP Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Whole Mount) (IHC (wm))

Güteklasse

KO Validated

Discover our top product VCP Primärantikörper

Bindungsspezifität
11

11

10

8

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term

Spezifität

Knockdown/Knockout validation was supported by customer review data.

Kreuzreaktivität

Human, Maus, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale

Rabbit Polyclonal antibody to VCP (valosin containing protein)
VCP antibody

Aufreinigung

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VCP. The exact sequence is proprietary.

Isotyp

IgG
anti-Valosin Containing Protein (VCP) (N-Term) antibody
VCP Reaktivität: Human WB, IF, IP, IHC (p), ICC, IHC (wm) Wirt: Kaninchen Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 125-371) antibody
VCP Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 647-806) antibody
VCP Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 1-588) antibody
VCP Reaktivität: Human WB, ELISA, IHC (p) Wirt: Maus Monoclonal 4A8 unconjugated

anti-Valosin Containing Protein (VCP) antibody
VCP Reaktivität: Human, Maus WB, FACS, ICC Wirt: Maus Monoclonal Hs-14 unconjugated

anti-Valosin Containing Protein (VCP) antibody
VCP Reaktivität: Human WB, IHC, ELISA, IF, IP Wirt: Kaninchen Monoclonal 5H12 unconjugated Recombinant Antibody

anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibody
VCP Reaktivität: Human, Maus, Ratte, Affe WB, IHC, IF, FACS, ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibody
VCP Reaktivität: Human, Maus, Ratte WB, IHC, IF, FACS, ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760) antibody
VCP Reaktivität: Human, Maus, Ratte WB, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (C-Term) antibody
VCP Reaktivität: Human, Maus, Ratte WB, IHC (p), ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare

Positive Control: Mouse brain , Rat brain
Validation: KO/KD, Orthogonal

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 mg/mL

Buffer

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Chen, Morales, Guerrero, Sun, McCarty: "PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells." in: Cancer research, Vol. 78, Issue 14, pp. 3809-3822, (2019) (PubMed).
Target

VCP (Valosin Containing Protein (VCP))

Andere Bezeichnung

valosin containing protein

Hintergrund

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Cellular Localization: Cytoplasm , cytosol , Nucleus

Molekulargewicht

89 kDa

Gen-ID

7415

UniProt

P55072

Pathways

ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway