CENPF Antikörper (C-Term)

Produktnummer ABIN2854654

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CENPF in WB, IHC (p), IF, ICC und IHC (wm). Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für CENPF Antikörper (C-Term) (ABIN2854654)

Target: CENPF (Centromere Protein F (CENPF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CENPF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Produktdetails anti-CENPF Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Human, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to CENPF (centromere protein F, 350/400 kDa (mitosin))
CENPF antibody [C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CENPF. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Raji

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-CENPF Antikörper (ABIN2854654)

Chen, Lin, Cheng, Lin, Lo, Yen, Lee, Hsiao: "Cdc6 cooperates with c-Myc to promote genome instability and epithelial to mesenchymal transition EMT in zebrafish." in: Oncotarget, Vol. 5, Issue 15, pp. 6300-11, (2015) (PubMed).

Details zu CENPF

Target: CENPF (Centromere Protein F (CENPF))

Andere Bezeichnung: centromere protein F

Hintergrund: This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.

Cellular Localization: Cytoplasm , perinuclear region , Nucleus matrix , Kinetochore , Spindle

Molekulargewicht: 368 kDa

Gen-ID: 1063

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion