RAD9A Antikörper (full length)

Produktnummer ABIN363404

Der Kaninchen Polyklonal Anti-RAD9A-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, RAD9A in Proben von Human zu detektieren.

Kurzübersicht für RAD9A Antikörper (full length) (ABIN363404)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD9A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-RAD9A Antikörper

Bindungsspezifität: full length

Spezifität: Reacts with human RAD9

Kreuzreaktivität (Details): Not tested in other species.

Aufreinigung: Antigen affinity purified

Immunogen: Fusion protein of full length human RAD9 protein

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- ICC/IF : 1/500 - WB : 1/100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: , Sodium azide 0.1 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Haltbarkeit: 6 months

Details zu RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Andere Bezeichnung: RAD9

Hintergrund: Rad9 (cell cycle checkpoint control protein RAD9A or hRAD9) is an evolutionarily conserved protein which serves multiple functions for preserving genomic integrity, and besides its role as intra-S/G2 checkpoint , it also implicates in homologous recombination repair, base excision repair and mismatch repair pathways. PRKCD mediated Rad9 phosphorylation is essential for the assembly of 9-1-1 cell-cycle checkpoint response complex which is recruited to DNA lesion upon damage by RFC (RAD17-replication factor C) clamp loader complex. Thereafter, Rad9 acts as sliding clamp platform on DNA for multiple proteins involved in LP-BER (long-patch base excision repair). 9-1-1 complex stimulates DNA polymerase beta activity/stability, and is necessary for the recruitment of RHNO1 to sites of DNA-DSBs that occurs during S phase of cell cycle. Moreover, Rad9 exerts 3'->5' double stranded DNA exonuclease activity, and it transactivate p53 target genes such as p21 and has been shown to induce apoptosis through its interaction with Bcl-2/Bcl-xL.

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus