EGFR Antikörper

Produktnummer ABIN1944913

Produktdetails anti-EGFR Antikörper

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. IHC: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C,-20 °C

Referenzen für anti-EGFR Antikörper (ABIN1944913)

Reiter, Threadgill, Eley, Strunk, Danielsen, Sinclair, Pearsall, Green, Yee, Lampland, Balasubramaniam, Crossley, Magnuson, James, Maihle: "Comparative genomic sequence analysis and isolation of human and mouse alternative EGFR transcripts encoding truncated receptor isoforms." in: Genomics, Vol. 71, Issue 1, pp. 1-20, (2001) (PubMed).

Ilekis, Gariti, Niederberger, Scoccia: "Expression of a truncated epidermal growth factor receptor-like protein (TEGFR) in ovarian cancer." in: Gynecologic oncology, Vol. 65, Issue 1, pp. 36-41, (1997) (PubMed).

Reiter, Maihle: "A 1.8 kb alternative transcript from the human epidermal growth factor receptor gene encodes a truncated form of the receptor." in: Nucleic acids research, Vol. 24, Issue 20, pp. 4050-6, (1996) (PubMed).

Ilekis, Stark, Scoccia: "Possible role of variant RNA transcripts in the regulation of epidermal growth factor receptor expression in human placenta." in: Molecular reproduction and development, Vol. 41, Issue 2, pp. 149-56, (1995) (PubMed).

Ullrich, Coussens, Hayflick, Dull, Gray, Tam, Lee, Yarden, Libermann, Schlessinger: "Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells." in: Nature, Vol. 309, Issue 5967, pp. 418-25, (1984) (PubMed).

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR (EGFR Produkte)

Hintergrund: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Molekulargewicht: 134277 Da

Gen-ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine