CD33 Antikörper (N-Term)

Produktnummer ABIN2798973

Dieses Anti-CD33-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CD33 in WB und IHC. Geeignet für Human.

Kurzübersicht für CD33 Antikörper (N-Term) (ABIN2798973)

Target: CD33

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD33 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-CD33 Antikörper

Bindungsspezifität: AA 12-39, N-Term

Verwendungszweck: Rabbit Anti-Human CD33 (SIGLEC3) (N-term) Antibody

Immunogen: This CD33 (SIGLEC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human CD33 (SIGLEC3).

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50The anti-Siglec3 N-term Pab , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.0 mg/mL

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C (short-term), -20°C (long-term)

Details zu CD33

Target: CD33

Andere Bezeichnung: CD33

Hintergrund:

Target Description: SIGLEC3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,6-linked sialic acid, the sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, SIGLEC3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. This protein Induces apoptosis in acute myeloid leukemia (in vitro). It has been shown to interact with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. SIGLEC3 expresses in monocytic/myeloid lineage cells, and contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. The gene for SIGLEC3 belongs to the immunoglobulin superfamily.

Gene Symbol: CD33

Molekulargewicht: 39825 Da

Gen-ID: 945

UniProt: P20138