Deoxyuridine Triphosphatase (DUT) (AA 170-198), (C-Term) Antikörper

Produktnummer ABIN2798480

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB und IHC validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Deoxyuridine Triphosphatase (DUT) (AA 170-198), (C-Term) Antikörper (ABIN2798480)

Target: Deoxyuridine Triphosphatase (DUT)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: AA 170-198, C-Term

Verwendungszweck: Rabbit Anti-Human DUT (C-term) Antibody

Immunogen: This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of lysate from A431 cell line, using DUT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C (short-term), -20°C (long-term)

Antigendetails

Target: Deoxyuridine Triphosphatase (DUT)

Andere Bezeichnung: DUT

Hintergrund:

Target Description: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Gene Symbol: DUT

Molekulargewicht: 26563 Da

Gen-ID: 1854

UniProt: P33316