p107 Antikörper (N-Term)

Produktnummer ABIN2792553

Dieses Anti-p107-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von p107 in WB. Geeignet für Human, Maus, Ratte, Rind (Kuh), Pferd, Schwein, Hund, Kaninchen und Meerschweinchen.

Kurzübersicht für p107 Antikörper (N-Term) (ABIN2792553)

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Schwein, Hund, Kaninchen, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p107 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-p107 Antikörper

Bindungsspezifität: N-Term

Sequenz: FLDIFQNPYE EPPKLPRSRK QRRIPCSVKD LFNFCWTLFV YTKGNFRMIG

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte (Rattus)

Homologie: Cow: 100%, Dog: 85%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 92%, Rat: 92%

Produktmerkmale: This is a rabbit polyclonal antibody against RBL1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RBL1

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-RBL1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: COLO205 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1014 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu p107

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Andere Bezeichnung: RBL1

Hintergrund: RBL1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: CP107, MGC40006, PRB1, p107
Protein Interaction Partner: DYRK1B, DYRK1A, CDK2, MAGEA11, E2F1, AR, UBC, RBBP8, E2F4, PLSCR1, NUCB1, LAMB2, NR4A1, GOLGA2, FN1, EPHA2, CDK6, CDK4, AOX1, SP1, SNRPD3, DGKZ, ID2, RINT1, E2F3, E2F2, PPP1CA, TOP1, SMARCA4, CCNA2, LIN9, LIN54, LIN37, MYBL2, MAPK6, IRF3, RBL2, DHX30, NR2
Protein Size: 1014

Molekulargewicht: 115 kDa

Gen-ID: 5933

NCBI Accession: NM_183404, NP_899662

Pathways: Zellzyklus, Mitotic G1-G1/S Phases