NFX1 Antikörper (Middle Region)

Produktnummer ABIN2792551

Der Kaninchen Polyklonal anti-NFX1 Antikörper wird verwendet zum Nachweis von NFX1 in Proben von Human, Maus, Ratte, Hund, Kaninchen und Rind (Kuh). Er wurde validiert für WB und IHC.

Kurzübersicht für NFX1 Antikörper (Middle Region) (ABIN2792551)

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-NFX1 Antikörper

Bindungsspezifität: Middle Region

Sequenz: TLTGVLEREM QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte (Rattus)

Homologie: Cow: 85%, Dog: 85%, Human: 100%, Mouse: 76%, Rabbit: 85%, Rat: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against NFX1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFX1

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1120 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu NFX1

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Andere Bezeichnung: NFX1

Hintergrund: MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.
Alias Symbols: DKFZp779G2416, MGC20369, NFX2
Protein Interaction Partner: UBC, BMI1, HECW2, SRSF4, SRSF3, RPS19, RPS3A, RPL22, RPL17, RPL13, RPL7A, RPL6, RPL3, PABPC3, YBX1, NCL, HNRNPM, HSPA1A, HNRNPU, HNRNPH1, HNRNPA2B1, HIST1H2AE, EEF1A2, DMD, DDX5, FAM208B, PABPC1, RPL13A, LARP1, APC2, MATR3, HIST1H4E, USP9X, SIN3A, MAML3,
Protein Size: 1120

Molekulargewicht: 124 kDa

Gen-ID: 4799

NCBI Accession: NM_002504, NP_002495

UniProt: Q12986