NFX1 Antikörper (C-Term)

Produktnummer ABIN6741622

Produktdetails anti-NFX1 Antikörper

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh), Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marke: IHC-plus™

Spezifität: Human NFX1

Homologie: Percent identity by BLAST analysis: Human (100%) Rat, Dog, Bovine, Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human NFX1 (Q12986, NP_002495). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Marmoset (100%), Gorilla, Galago, Pig, Guinea pig (92%), Rat, Dog, Bovine, Rabbit (85%), Mouse (76%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NFX1

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Andere Bezeichnung: NFX1 (NFX1 Produkte)

Synonyme: NFX1 antikoerper, NFX2 antikoerper, 1300017N15Rik antikoerper, 3000003M19Rik antikoerper, NFX.1 antikoerper, TEG-42 antikoerper, Tex42 antikoerper, nuclear transcription factor, X-box binding 1 antikoerper, nuclear transcription factor, x-box binding 1 antikoerper, NFX1 antikoerper, CpipJ_CPIJ011986 antikoerper, nfx1 antikoerper, Nfx1 antikoerper

Hintergrund: Name/Gene ID: NFX1

Synonyms: NFX1, NFX2

Gen-ID: 4799

NCBI Accession: NP_002495

UniProt: Q12986