PRAME Antikörper (N-Term)

Produktnummer ABIN2786465

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-PRAME Antikörper (ABIN2786465) detektiert spezifisch PRAME in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh), Pferd und Schwein.

Kurzübersicht für PRAME Antikörper (N-Term) (ABIN2786465)

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRAME Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PRAME Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: PRAME Antibody - N-terminal region

Sequenz: MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR

Homologie: Cow: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 86%, Rat: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against PRAME. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRAME

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55981-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-PRAME Antikörper (ABIN2786465)

Liu, Zhao, Lu, Ning, Ma, Diaz, OConnor: "A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle." in: Reproduction (Cambridge, England), Vol. 153, Issue 6, pp. 847-863, (2017) (PubMed).

Details zu PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Andere Bezeichnung: PRAME

Hintergrund:

Background Information: PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. PRAME prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.

Gene Name: Preferentially expressed antigen in melanoma

Alternative Symbols: MAPE, OIP4, CT130, OIP-4

Protein Name: Melanoma antigen preferentially expressed in tumors

Molekulargewicht: 56kDa

Gen-ID: 23532

NCBI Accession: NP_006106

UniProt: P78395

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding