IDH1 Antikörper (C-Term)

Produktnummer ABIN2785883

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-IDH1 Antikörper wird verwendet zum Nachweis von IDH1 in Proben von Human, Maus, Ratte, Hund, Pferd, Meerschweinchen, Rind (Kuh), Kaninchen und Schaf. Er wurde validiert für WB und IHC.

Kurzübersicht für IDH1 Antikörper (C-Term) (ABIN2785883)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Meerschweinchen, Rind (Kuh), Kaninchen, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IDH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-IDH1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: IDH1 Antibody - C-terminal region

Sequenz: VSIETIEAGF MTKDLAACIK GLPNVQRSDY LNTFEFMDKL GENLKIKLAQ

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against IDH1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human IDH1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54788-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-IDH1 Antikörper (ABIN2785883)

Jena, Janjanam, Naru, Kumar, Kumar, Singh, Mohapatra, Kola, Anand, Jaswal, Verma, Malakar, Dang, Kaushik, Reddy, Mohanty: "DIGE based proteome analysis of mammary gland tissue in water buffalo (Bubalus bubalis): Lactating vis-a-vis heifer." in: Journal of proteomics, Vol. 119C, pp. 100-111, (2015) (PubMed).

Details zu IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Andere Bezeichnung: IDH1

Hintergrund:

Background Information: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+).The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Isocitrate dehydrogenase 1 (NADP+), soluble

Alternative Symbols: IDH, IDP, IDCD, IDPC, PICD, HEL-216, HEL-S-26

Protein Name: Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial

Molekulargewicht: 47kDa

Gen-ID: 3417

NCBI Accession: NP_005887

UniProt: O75874

Pathways: Warburg Effekt