ETF1 Antikörper (N-Term)

Produktnummer ABIN2785859

Der Kaninchen Polyklonal anti-ETF1 Antikörper wird verwendet zum Nachweis von ETF1 in Proben von Human, Maus, Ratte, Zebrafisch (Danio rerio), Rind (Kuh), Hund, Meerschweinchen, Kaninchen, Pferd und Saccharomyces cerevisiae. Er wurde validiert für WB und IHC.

Kurzübersicht für ETF1 Antikörper (N-Term) (ABIN2785859)

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Rind (Kuh), Hund, Meerschweinchen, Kaninchen, Pferd, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ETF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ETF1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: ETF1 Antibody - N-terminal region

Sequenz: ISLIIPPKDQ ISRVAKMLAD EFGTASNIKS RVNRLSVLGA ITSVQQRLKL

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against ETF1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ETF1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54738-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Andere Bezeichnung: ETF1

Hintergrund:

Targeted Subunit: 1

Background Information: Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).

Gene Name: Eukaryotic translation termination factor 1

Alternative Symbols: ERF, RF1, ERF1, TB3-1, D5S1995, SUP45L1

Protein Name: Eukaryotic peptide chain release factor subunit 1

Molekulargewicht: 49kDa

Gen-ID: 2107

NCBI Accession: NP_004721

UniProt: P62495