ETF1 Antikörper (C-Term)

Produktnummer ABIN7466955

Produktdetails anti-ETF1 Antikörper

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ETF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eRF1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.91 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Andere Bezeichnung: eukaryotic translation termination factor 1 (ETF1 Produkte)

Synonyme: D5S1995 antikoerper, ERF antikoerper, ERF1 antikoerper, RF1 antikoerper, SUP45L1 antikoerper, TB3-1 antikoerper, AI463371 antikoerper, D6Ertd109e antikoerper, eRF1 antikoerper, zeh0057 antikoerper, hm:zeh0057 antikoerper, wu:fa19e07 antikoerper, wu:fi31f01 antikoerper, cl1 antikoerper, RRF1 antikoerper, DDBDRAFT_0188023 antikoerper, DDBDRAFT_0191343 antikoerper, DDB_0188023 antikoerper, DDB_0191343 antikoerper, CG5605 antikoerper, Dm0184 antikoerper, Dmel\\CG5605 antikoerper, Group J antikoerper, group J antikoerper, l(3)00103 antikoerper, l(3)05637 antikoerper, l(3)77ABb antikoerper, l(3)neo28 antikoerper, Eukaryotic release factor 1 antikoerper, eukaryotic translation termination factor 1 antikoerper, eukaryotic translation termination factor 1b antikoerper, eukaryotic translation termination factor 1 S homeolog antikoerper, eukaryotic peptide chain release factor subunit 1 antikoerper, eukaryotic petide chain release factor subunit 1 antikoerper, eukaryotic release factor 1 antikoerper, Eukaryotic peptide chain release factor subunit 1 antikoerper, ETF1 antikoerper, Etf1 antikoerper, etf1b antikoerper, etf1 antikoerper, etf1.S antikoerper, Tb11.22.0012 antikoerper, THAPSDRAFT_22240 antikoerper, TTHERM_00292170 antikoerper, LOC692937 antikoerper, erf1 antikoerper, eRF1 antikoerper, erfa-1 antikoerper

Hintergrund: Eukaryotic translation termination factor 1 , D5S1995 , ERF , ERF1 , RF1 , SUP45L1 , TB3-1,Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Molekulargewicht: 49 kDa

Gen-ID: 2107

UniProt: P62495