GCLM Antikörper (Middle Region)

Produktnummer ABIN2785805

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch GCLM in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund, Meerschweinchen, Pferd und Zebrafisch (Danio rerio) und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für GCLM Antikörper (Middle Region) (ABIN2785805)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund, Meerschweinchen, Pferd, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GCLM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GCLM Antikörper

Bindungsspezifität: Middle Region

Sequenz: KPNSNQVNLA SCCVMPPDLT AFAKQFDIQL LTHNDPKELL SEASFQEALQ

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Produktmerkmale: This is a rabbit polyclonal antibody against GCLM. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLM

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 274 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-GCLM Antikörper (ABIN2785805)

Tomasi, Ryoo, Yang, Iglesias Ara, Ko, Lu: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." in: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).

Ramani, Tomasi, Yang, Ko, Lu: "Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 36341-55, (2012) (PubMed).

Details zu GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Andere Bezeichnung: GCLM

Hintergrund: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GLCLR
Protein Interaction Partner: UBC, NAGK, GLRX3, GNAI2, GCLC, STOX1, IRF7, CALM1, SORD, CUL3,
Protein Size: 274

Molekulargewicht: 31 kDa

Gen-ID: 2730

NCBI Accession: NM_002061, NP_002052

UniProt: P48507