MED25 Antikörper (N-Term)

Produktnummer ABIN567247

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch MED25 in WB und ChIP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Schwein, Meerschweinchen und Zebrafisch (Danio rerio) und wurde in 1 Publikation erwähnt.

Kurzübersicht für MED25 Antikörper (N-Term) (ABIN567247)

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein, Meerschweinchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-MED25 Antikörper

Bindungsspezifität: N-Term

Sequenz: EGLRKHYLLP AIEYFNGGPP AETDFGGDYG GTQYSLVVFN TVDCAPESYV

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 85%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against MED25. It was validated on Western Blot.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED25

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 747 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-MED25 Antikörper (ABIN567247)

Sela, Conkright, Chen, Gilmore, Washburn, Florens, Conaway, Conaway: "Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6?." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26179-87, (2013) (PubMed).

Details zu MED25

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Andere Bezeichnung: MED25

Hintergrund: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Alias Symbols: ACID1, ARC92, CMT2B2, DKFZp434K0512, MGC70671, P78, PTOV2, TCBAP0758
Protein Interaction Partner: UBC, MED19, MED26, EPAS1, NOTCH1, DREB2A, CTDP1, MED6, THRA, RXRA, RARA, MED1, NR3C1, ESR1, CREBBP, SREBF1, MED25, MED15, MED16, MED13, MED12, MED7, MED17, MED23, MED14, CDK8, ZC3H13, QKI, TRIP4, TADA2A, MED28, OBFC1, TRRAP, UL48, MED9, MED8, HCFC1, MED10
Protein Size: 747

Molekulargewicht: 78 kDa

Gen-ID: 81857

NCBI Accession: NM_030973, NP_112235

UniProt: Q71SY5

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response