Mediator Complex Subunit 25 (MED25) (N-Term) Antikörper

Details zu Produkt Nr. ABIN2784291, Anbieter: Anmelden zum Anzeigen
Antigen
  • Arc92
  • CG12254
  • Dmel\\CG12254
  • LD07688
  • Med25
  • dARC92
  • dDrip97
  • med25
  • ACID1
  • ARC92
  • CMT2B2
  • P78
  • PTOV2
  • RGD1305137
  • 2610034E13Rik
  • 2610529E18Rik
  • ESTM2
  • X83308
  • zgc:63555
  • Mediator complex subunit 25
  • mediator complex subunit 25
  • mediator complex subunit 25 S homeolog
  • MED25
  • med25
  • Med25
  • med25.S
Epitop
N-Term
11
10
9
3
1
Reaktivität
Rind (Kuh), Hund, Meerschweinchen, Human, Maus, Schwein, Ratte (Rattus), Zebrafisch (Danio rerio)
24
16
3
3
3
3
2
1
1
1
1
1
1
1
Wirt
Kaninchen
24
Klonalität
Polyklonal
Konjugat
Unkonjugiert
2
2
2
2
2
2
Applikation
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
23
14
14
4
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MED25
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN979947.
Sequenz EGLRKHYLLP AIEYFNGGPP AETDFGGDYG GTQYSLVVFN TVDCAPESYV
Homologie Cow: 100%, Dog: 100%, Guinea Pig: 85%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%
Produktmerkmale This is a rabbit polyclonal antibody against MED25. It was validated on Western Blot.
Reinigung Affinity Purified
Andere Bezeichnung MED25 (MED25 Antibody Abstract)
Hintergrund This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Alias Symbols: ACID1, ARC92, CMT2B2, DKFZp434K0512, MGC70671, P78, PTOV2, TCBAP0758
Protein Interaction Partner: UBC, MED19, MED26, EPAS1, NOTCH1, DREB2A, CTDP1, MED6, THRA, RXRA, RARA, MED1, NR3C1, ESR1, CREBBP, SREBF1, MED25, MED15, MED16, MED13, MED12, MED7, MED17, MED23, MED14, CDK8, ZC3H13, QKI, TRIP4, TADA2A, MED28, OBFC1, TRRAP, UL48, MED9, MED8, HCFC1, MED10
Protein Size: 747
Molekulargewicht 78 kDa
Gen-ID 81857
NCBI Accession NM_030973, NP_112235
UniProt Q71SY5
Pathways Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response
Applikationshinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 747 AA

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) WB Suggested Anti-MED25 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) WB Suggested Anti-MED25
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
Produkt verwendet in: Sela, Conkright, Chen, Gilmore, Washburn, Florens, Conaway, Conaway: "Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6?." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26179-87, 2013 (PubMed).

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