SIGLEC12 Antikörper (N-Term)

Produktnummer ABIN2784083

Der Kaninchen Polyklonal anti-SIGLEC12 Antikörper (ABIN2784083) detektiert spezifisch SIGLEC12 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Rind (Kuh), Pferd, Schwein und Zebrafisch (Danio rerio).

Kurzübersicht für SIGLEC12 Antikörper (N-Term) (ABIN2784083)

Target: SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))

Reaktivität: Human, Ratte, Rind (Kuh), Pferd, Schwein, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIGLEC12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SIGLEC12 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: SIGLEC12 Antibody - N-terminal region

Sequenz: GRFLLLGDPQ TNNCSLSIRD ARKGDSGKYY FQVERGSRKW NYIYDKLSVH

Homologie: Cow: 77%, Horse: 83%, Human: 100%, Pig: 86%, Rat: 77%, Zebrafish: 82%

Produktmerkmale: This is a rabbit polyclonal antibody against SIGLEC12. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SIGLEC12

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP50183-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu SIGLEC12

Target: SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))

Andere Bezeichnung: SIGLEC12

Hintergrund:

Background Information: Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. SIGLEC12 is a member of the SIGLEC3-like subfamily of SIGLECs. SIGLEC12, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor.Western blots using four different antibodies against four unique regions of this protein target confirm the same apparent molecular weight in our tests.Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.

Gene Name: Sialic acid binding Ig-like lectin 12 (gene/pseudogene)

Alternative Symbols: S2V, SLG, SIGLECL1, Siglec-XII

Protein Name: Sialic acid-binding Ig-like lectin 12

Molekulargewicht: 63kDa

Gen-ID: 89858

NCBI Accession: NP_443729

UniProt: Q96PQ1