DNMT3B Antikörper (Middle Region)

Produktnummer ABIN2783648

Produktdetails anti-DNMT3B Antikörper

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNMT3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: GTGRLFFEFY HLLNYSRPKE GDDRPFFWMF ENVVAMKVGD KRDISRFLEC

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against DNMT3B. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DNMT3B

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 770 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu DNMT3B

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Andere Bezeichnung: DNMT3B (DNMT3B Produkte)

Hintergrund: DNMT3B is required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. DNMT3B may preferentially methylate nucleosomal DNA within the nucleosome core region. DNMT3B may function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Alias Symbols: ICF, M.HsaIIIB, ICF1
Protein Interaction Partner: RBBP7, RBBP4, HDAC2, HDAC1, CHD4, MTA2, MTA1, POLR2H, POLR2C, POLR2A, DNMT1, LEO1, CDC73, PAF1, RTF1, CTR9, NRIP1, EZH2, DNMT3L, SUZ12, SIRT1, EED, UBC, EPHB1, CREB1, CBX5, TRIM28, ZFP57, UHRF2, UHRF1, MAP1LC3B, UBE2W, PLEKHJ1, DUSP23, CMTM6, PAM16, TSC22
Protein Size: 770

Molekulargewicht: 86 kDa

Gen-ID: 1789

NCBI Accession: NM_175849, NP_787045