RRM1 Antikörper (C-Term)

Produktnummer ABIN2782438

Der Kaninchen Polyklonal Anti-RRM1-Antikörper wurde für WB validiert. Er ist geeignet, RRM1 in Proben von Human, Maus, Ratte, Zebrafisch (Danio rerio), Hund, Kaninchen, Pferd, Meerschweinchen und Rind (Kuh) zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für RRM1 Antikörper (C-Term) (ABIN2782438)

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Hund, Kaninchen, Pferd, Meerschweinchen, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RRM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RRM1 Antikörper

Bindungsspezifität: C-Term

Sequenz: MHFYGWKQGL KTGMYYLRTR PAANPIQFTL NKEKLKDKEK VSKEEEEKER

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against RRM1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RRM1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 792 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-RRM1 Antikörper (ABIN2782438)

Zhu, Wonganan, Lansakara-P, OMary, Li, Cui: "The effect of the acid-sensitivity of 4-(N)-stearoyl gemcitabine-loaded micelles on drug resistance caused by RRM1 overexpression." in: Biomaterials, Vol. 34, Issue 9, pp. 2327-39, (2013) (PubMed).

Chung, Sandoval, Sloat, Lansakara-P, Cui: "Stearoyl gemcitabine nanoparticles overcome resistance related to the over-expression of ribonucleotide reductase subunit M1." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 157, Issue 1, pp. 132-40, (2012) (PubMed).

Wonganan, Chung, Zhu, Kiguchi, Digiovanni, Cui: "Silencing of ribonucleotide reductase subunit M1 potentiates the antitumor activity of gemcitabine in resistant cancer cells." in: Cancer biology & therapy, Vol. 13, Issue 10, pp. 908-14, (2012) (PubMed).

Details zu RRM1

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Andere Bezeichnung: RRM1

Hintergrund: RRM1 is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. Its gene is located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. Its gene may play a role in malignancies and disease that involve this region.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.
Alias Symbols: R1, RIR1, RR1
Protein Interaction Partner: UBC, RRM1, SUMO2, RNF2, BMI1, PPIB, TNPO1, FBXO6, PPP1CC, TMEM177, DNAJB11, SUCLA2, SGSH, NDUFV1, EEF2, DNASE2, COPS5, CDK2, RRM2, RRM2B, KAT5, LRIF1, YWHAG,
Protein Size: 792

Molekulargewicht: 90 kDa

Gen-ID: 6240

NCBI Accession: NM_001033, NP_001024

UniProt: P23921

Pathways: Positive Regulation of Endopeptidase Activity