Deoxyuridine Triphosphatase (DUT) (N-Term) Antikörper

Produktnummer ABIN2782437

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-Deoxyuridine Triphosphatase Antikörper wird verwendet zum Nachweis von Deoxyuridine Triphosphatase in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für Deoxyuridine Triphosphatase (DUT) (N-Term) Antikörper (ABIN2782437)

Target: Deoxyuridine Triphosphatase (DUT)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: N-Term

Verwendungszweck: DUT Antibody - N-terminal region

Sequenz: AAVLSGPGPP LGRAAQHGIP RPLSSAGRLS QGCRGASTVG AAGWKGELPK

Homologie: Human: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against DUT. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DUT

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46028-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen

Tóth, Varga, Kovács, Málnási-Csizmadia, Vértessy: "Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme." in: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33572-82, (2007) (PubMed).

Antigendetails

Target: Deoxyuridine Triphosphatase (DUT)

Andere Bezeichnung: DUT

Hintergrund:

Background Information: DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Gene Name: Deoxyuridine triphosphatase

Alternative Symbols: dUTPase

Protein Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Molekulargewicht: 19kDa

Gen-ID: 1854

NCBI Accession: NP_001020419

UniProt: P33316