Deoxyuridine Triphosphatase (DUT) (C-Term) Antikörper

Produktnummer ABIN2782436

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Hund, Rind (Kuh), Meerschweinchen, Pferd, Saccharomyces cerevisiae, Zebrafisch (Danio rerio) und Kaninchen und wurde in 1 Publikation erwähnt.

Kurzübersicht für Deoxyuridine Triphosphatase (DUT) (C-Term) Antikörper (ABIN2782436)

Target: Deoxyuridine Triphosphatase (DUT)

Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Meerschweinchen, Pferd, Saccharomyces cerevisiae, Zebrafisch (Danio rerio), Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: C-Term

Sequenz: NFGKEKFEVK KGDRIAQLIC ERIFYPEIEE VQALDDTERG SGGFGSTGKN

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Yeast: 93%, Zebrafish: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against DUT. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DUT

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 252 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen

Studebaker, Lafuse, Kloesel, Williams: "Modulation of human dUTPase using small interfering RNA." in: Biochemical and biophysical research communications, Vol. 327, Issue 1, pp. 306-10, (2005) (PubMed).

Antigendetails

Target: Deoxyuridine Triphosphatase (DUT)

Andere Bezeichnung: DUT

Hintergrund: DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Alias Symbols: FLJ20622, dUTPase
Protein Interaction Partner: GDI2, NUDT18, PLEKHF2, C19orf25, MRPL14, LEMD3, UBL4A, RPL38, UBC, CUL3, ESRRG, ESRRA, ESR1, SPATA2, DUT, PPARD, PPARA, CDK1,
Protein Size: 252

Molekulargewicht: 19 kDa

Gen-ID: 1854

NCBI Accession: NM_001025248, NP_001020419

UniProt: P33316