SETDB1 Antikörper (N-Term)

Produktnummer ABIN2780883

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SETDB1 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd und Kaninchen.

Kurzübersicht für SETDB1 Antikörper (N-Term) (ABIN2780883)

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Reaktivität: Human, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SETDB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SETDB1 Antikörper

Bindungsspezifität: N-Term

Sequenz: PGCIGLDAAT ATVESEEIAE LQQAVVEELG ISMEELRHFI DEELEKMDCV

Homologie: Cow: 92%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Rabbit: 85%, Rat: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against SETDB1. It was validated on Western Blot.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETDB1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu SETDB1

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Andere Bezeichnung: SETDB1

Hintergrund: SETDB1 is a histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Protein Interaction Partner: MDM2, HSP90AA1, UBC, SKIL, LRIF1, BARD1, APLP1, TCERG1, TRIM28, VIM, SUMO2, PML, CLSTN1, JARID2, tat, MZT2B, FAM118B, MRPL44, LUC7L2, MAP4K5, TSC22D1, TTR, TK1, SMN1, PCYT2, PSME1, GRB7, CDKN1A, CDK4, BID, ANXA7, ZNF274, HIST1H3A, ESCO1, AKT2, AKT1, PPARG
Protein Size: 397

Molekulargewicht: 43 kDa

Gen-ID: 9869