SETDB1 Antikörper (N-Term)

Produktnummer ABIN2780883

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SETDB1 in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd und Kaninchen.

Kurzübersicht für SETDB1 Antikörper (N-Term) (ABIN2780883)

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Reaktivität: Human, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SETDB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SETDB1 Antikörper

Bindungsspezifität: N-Term

Sequenz: PGCIGLDAAT ATVESEEIAE LQQAVVEELG ISMEELRHFI DEELEKMDCV

Homologie: Cow: 92%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Rabbit: 85%, Rat: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against SETDB1. It was validated on Western Blot.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETDB1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu SETDB1

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Andere Bezeichnung: SETDB1

Hintergrund: SETDB1 is a histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Protein Interaction Partner: MDM2, HSP90AA1, UBC, SKIL, LRIF1, BARD1, APLP1, TCERG1, TRIM28, VIM, SUMO2, PML, CLSTN1, JARID2, tat, MZT2B, FAM118B, MRPL44, LUC7L2, MAP4K5, TSC22D1, TTR, TK1, SMN1, PCYT2, PSME1, GRB7, CDKN1A, CDK4, BID, ANXA7, ZNF274, HIST1H3A, ESCO1, AKT2, AKT1, PPARG
Protein Size: 397

Molekulargewicht: 43 kDa

Gen-ID: 9869