E2F2 Antikörper (Middle Region)

Produktnummer ABIN2780568

Der Kaninchen Polyklonal Anti-E2F2-Antikörper wurde für WB und IHC validiert. Er ist geeignet, E2F2 in Proben von Human, Maus, Ratte, Kaninchen, Hund, Rind (Kuh), Meerschweinchen, Pferd und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für E2F2 Antikörper (Middle Region) (ABIN2780568)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reaktivität: Human, Maus, Ratte, Kaninchen, Hund, Rind (Kuh), Meerschweinchen, Pferd, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-E2F2 Antikörper

Bindungsspezifität: Middle Region

Sequenz: DQFLSPTLAC SSPLISFSPS LDQDDYLWGL EAGEGISDLF DSYDLGDLLI

Homologie: Cow: 92%, Dog: 100%, Guinea Pig: 91%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 75%

Produktmerkmale: This is a rabbit polyclonal antibody against E2F2. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human E2F2

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 437 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Andere Bezeichnung: E2F2

Hintergrund: E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: E2F-2
Protein Interaction Partner: RB1, GNB5, GIT2, TFDP1, RBL1, ARID3A, ELAVL1, ATAD2, KMT2D, KMT2A, RYBP, SETD8, BCAR1, TFDP2, BRD2, YY1, SP1, CDK3, RNF144A, FHL2, SPIB, RBL2, E2F4, MYBL2, EAPP, TP53BP2, PPP1R13B, TP53INP1, JMY, GAB2, CDC25A, CDC6, E2F1, UXT, MT1G,
Protein Size: 437

Molekulargewicht: 47 kDa

Gen-ID: 1870

NCBI Accession: NM_004091, NP_004082

UniProt: Q14209

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication