HES1 Antikörper (N-Term)

Produktnummer ABIN2779597

Der Kaninchen Polyklonal Anti-HES1-Antikörper wurde für WB, IHC, IF und IP validiert. Er ist geeignet, HES1 in Proben von Human, Maus, Ratte, Rind (Kuh), Kaninchen, Meerschweinchen, Zebrafisch (Danio rerio) und Pferd zu detektieren. Es sind 13+ Publikationen verfügbar.

Kurzübersicht für HES1 Antikörper (N-Term) (ABIN2779597)

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Meerschweinchen, Zebrafisch (Danio rerio), Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HES1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-HES1 Antikörper

Bindungsspezifität: N-Term

Sequenz: NTTPDKPKTA SEHRKSSKPI MEKRRRARIN ESLSQLKTLI LDALKKDSSR

Homologie: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against HES1. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HES1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 280 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-HES1 Antikörper (ABIN2779597)

Zhang, Weimann, Stolzenburg, Neuhaus, Berndt-Paetz: "Notch2/3-DLL4 interaction in urothelial cancer cell lines supports a tumorigenic role of Notch signaling pathways in bladder carcinoma." in: PloS one, Vol. 20, Issue 2, pp. e0317709, (2025) (PubMed).

Sun, Zhou, Liu, Zhang, Chen, Pan, Ma, Liu, Du, Yang, Wang: "Inhibition of breast cancer cell survival by Xanthohumol via modulation of the Notch signaling pathway in vivo and in vitro." in: Oncology letters, Vol. 15, Issue 1, pp. 908-916, (2018) (PubMed).

Natsumeda, Maitani, Liu, Miyahara, Kaur, Chu, Zhang, Kahlert, Eberhart: "Targeting Notch Signaling and Autophagy Increases Cytotoxicity in Glioblastoma Neurospheres." in: Brain pathology (Zurich, Switzerland), Vol. 26, Issue 6, pp. 713-723, (2015) (PubMed).

Meng, Su, Liu, Wang, Wang: "Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2." in: Neuroscience, Vol. 306, pp. 100-14, (2015) (PubMed).

Ma, Mao, Shen, Zheng, Li, Liu, Ni: "Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 353-9, (2014) (PubMed).

Asnaghi, Lin, Lim, Lim, Tripathy, Wendeborn, Merbs, Handa, Sodhi, Bar, Eberhart: "Hypoxia promotes uveal melanoma invasion through enhanced Notch and MAPK activation." in: PLoS ONE, Vol. 9, Issue 8, pp. e105372, (2014) (PubMed).

Guiu, Shimizu, DAltri, Fraser, Hatakeyama, Bresnick, Kageyama, Dzierzak, Yamamoto, Espinosa, Bigas: "Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling." in: The Journal of experimental medicine, Vol. 210, Issue 1, pp. 71-84, (2013) (PubMed).

Chu, Liu, Wang: "Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner." in: Cell proliferation, Vol. 46, Issue 3, pp. 254-62, (2013) (PubMed).

Asnaghi, Ebrahimi, Schreck, Bar, Coonfield, Bell, Handa, Merbs, Harbour, Eberhart: "Notch signaling promotes growth and invasion in uveal melanoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 18, Issue 3, pp. 654-65, (2012) (PubMed).

Ma, Du, Chu, Wang, Liu, Song, Wang: "hCLP46 regulates U937 cell proliferation via Notch signaling pathway." in: Biochemical and biophysical research communications, Vol. 408, Issue 1, pp. 84-8, (2011) (PubMed).

Bennani-Baiti, Aryee, Ban, Machado, Kauer, Mühlbacher, Amann, Llombart-Bosch, Kovar: "Notch signalling is off and is uncoupled from HES1 expression in Ewing's sarcoma." in: The Journal of pathology, Vol. 225, Issue 3, pp. 353-63, (2011) (PubMed).

Schreck, Taylor, Marchionni, Gopalakrishnan, Bar, Gaiano, Eberhart: "The Notch target Hes1 directly modulates Gli1 expression and Hedgehog signaling: a potential mechanism of therapeutic resistance." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 24, pp. 6060-70, (2010) (PubMed).

Tilley, Harvey, Heguy, Hackett, Wang, OConnor, Crystal: "Down-regulation of the notch pathway in human airway epithelium in association with smoking and chronic obstructive pulmonary disease." in: American journal of respiratory and critical care medicine, Vol. 179, Issue 6, pp. 457-66, (2009) (PubMed).

Details zu HES1

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Andere Bezeichnung: HES1

Hintergrund: HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Alias Symbols: HHL, HRY, HES-1, bHLHb39
Protein Interaction Partner: STAT3, JAK2, APH1A, LTBR, ASGR2, TLE1, ELAVL1, PARP1, UBC, NUDT3, HDAC6, YWHAB, PTK2, NR4A1, HMGCL, GAPDH, FHL1, CSNK1E, APCS, FANCE, FANCC, FANCA, FANCL, SPTAN1, HSP90AA1, HSPA4, FANCG, FANCF, HDAC1, RUNX2, HES6, ID4, ID1, SIRT1, UBQLN1, HEY2, ID2, ID3,
Protein Size: 280

Molekulargewicht: 30 kDa

Gen-ID: 3280

NCBI Accession: NM_005524, NP_005515

UniProt: Q14469

Pathways: DNA Reparatur